ENZO熱銷產品——ER-ID® Green assay kit內質網檢測試劑盒（綠色）
Enzo Life Sciences公司的ER-ID® Green assay kit適用于活細胞或破膜醛固定細胞的染色。微摩爾級濃度的ER-ID®綠色染料足以對哺乳動物細胞進行染色，在人宮頸癌細胞系、T淋巴細胞系、Jurkat 細胞系、HeLa 細胞系以及骨肉瘤上皮細胞U2-OS中已經得到了驗證。ER-ID® Green assay kit專門用于表達RFP或OFP的細胞系，以及表達藍色或青色熒光蛋白（BFP、CFP）的細胞。該試劑盒適用于活細胞或固定后細胞與探針（如標記抗體）或與Texas Red或香-豆-素類似光譜性質的其他熒光綴合物結合使用。
產品特點
● 新型內質網染料，可用于活細胞、破膜細胞或固定細胞的染色
● 與常見熒光染料和熒光蛋白兼容
● 高耐光漂白性、抗濃度淬滅和光轉換
● 生產嚴格，以控制和消除非特異性結果
如需購買Enzo Life Sciences公司產品，或咨詢產品技術問題，請聯系代理商欣博盛生物。
實例分析
● ER-ID®綠色染色與含有KDEL序列的鈣網蛋白共定位，與橙色熒光蛋白（OFP）融合。
● 用ER-ID®綠色染料（A）、Hoechst 33342染料（B）對HeLa活細胞進行染色，得到的合成圖像（C）。
● 用4%多聚甲醛固定HepG2細胞20分鐘，并用ER-ID®標記30分鐘。下圖為標記3天后拍攝的照片。
● 將HepG2細胞與ER-ID®孵育30分鐘，并用倒置顯微鏡（Zeiss）進行分析。
產品詳情：
產品名稱 | ER-ID® Green assay kit |
產品貨號 | ENZ-51025-K500 |
產品規格 | 500 assays |
質量控制 | A sample from each lot of ER-ID® Green assay kit is used to stain HeLa cells, using the procedures described in the user manual. The selectivity of the ER-ID® Green dye is evident. |
應用 | Fluorescence microscopy Enzo Life Sciences’ ER-ID® Green assay kit contains a novel endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. |
長期儲存 | -20°C |
組分 | ER-ID® Green detection reagent: 50μl Hoechst 33342 nuclear stain: 50μl 10X assay buffer: 15ml |
產品說明 | The ER-ID® Green assay kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents |
部分產品文獻引用
1. Molecular interplay between NOX1 and autophagy in cadmium-induced prostate carcinogenesis: A. Tyagi, et al.; Free Radic. Biol. Med. 199, 44 (2023)
2. Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum: A. Komatsu, et al.; J. Biol. Chem. 298, 101933 (2022)
3. PLP1 mutations in patients with multiple sclerosis: identification of a new mutation and potential pathogenicity of the mutations: N.C. Cloake, et al.; J. Clin. Med. 7, 342 (2018), Application(s): Fluorescence microscopy
4. Englerin A induces an acute inflammatory response and reveals lipid metabolism and ER stress as targetable vulnerabilities in renal cell carcinoma: A. Batova, et al.; PLoS One 12, e0172632 (2017), Application(s): Fluorescence microscopy
5. Cell growth on (“Janus") density gradients of bifunctional zeolite L crystals: N.S. Kehr, et al.; ACS Appl. Mater. Interfaces 8, 35081 (2016)
6. The parathyroid hormone second receptor PTH2R and its ligand tuberoinfundibular peptide of 39 residues TIP39 regulate intracellular calcium and influence keratinocyte differentiation: E. Sato, et al.; J. Invest. Dermatol. 136, 1449 (2016)
7. Viral genome imaging of hepatitis C virus to probe heterogeneous viral infection and responses to antiviral therapies: V. Ramanan, et al.; Virology 494, 236 (2016), Application(s): Endoplasmic reticulum staining
8. Localization-Dependent Cell-Killing Effects of Protoporphyrin (PPIX)-Lipid Micelles and Liposomes in Photodynamic Therapy: S. Tachikawa, et al.; Bioorg. Med. Chem. 23, 7578 (2015), Application(s): Cell staining
詳情請聯系 ENZO 金牌代理商-欣博盛生物科技
在線咨詢
電話咨詢
