GAPDH抗體識別甘油醛-3-磷酸脫氫酶(GAPDH)蛋白，相基因的產物催化碳水化合物代謝中一個重要的能量產生步驟，即在無機磷酸鹽和煙酰胺腺嘌呤二核苷酸（NAD）存在的情況下，對甘油醛-3-磷酸進行可逆的氧化磷酸化。GeneTex熱銷抗體 —— GAPDH抗體（克隆號GT239）助理您的科研實驗！
產品詳情
產品名稱：GAPDH antibody [GT239]
別名：glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP
反應種屬：Candida albicans, Chinese Hamster, Dog, Drosophila, E. coli, Hamster, Human, Monkey, Mouse, Pig, Rat, Yeast, Zebrafish
宿主來源：Mouse
實驗應用：EMSA, ICC/IF, IHC-P, WB
同種型：IgG2b
免疫原：Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
克隆性：Monoclonal
克隆號：GT239
純化方式：Affinity purified by Protein G.
偶聯：Unconjugated
產品濃度：1 mg/ml (Please refer to the vial label for the specific concentration.)
保存溫度：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4oC. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
運輸溫度：4°C
預期分子量：36 kDa
產品形式：Liquid
存儲溶液：PBS, no preservative.
驗證數據
| WB ImageGAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 30 μg yeast whole cell lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] (GTX627408) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. |
| ICC/IF：GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:500. Blue: Hoechst 33342 staining. |
| IHC-P：GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
產品部分引用文獻
1.S Shivaprasad et al. Viruses 2024; 16 (5) : 725 CRISPR Screen Reveals PACT as a Pro-Viral Factor for Dengue Viral Replication.
2.X Chen et al. Nature 2024; 628 (8009) : 818-825 Antisense oligonucleotide therapeutic approach for Timothy syndrome.
3.CP Chen et al. Heliyon 2024; 10 (7) : 29055 Synergistic effects of the combination of trametinib and alpelisib in anaplastic thyroid cancer with BRAF and PI3KCA co-mutations.
4.YL Chen et al. Viruses 2024; 16 : 523 Novel Anti-Viral Properties of the Herbal Extract of Davallia mariesii against Influenza A Virus.
訂購詳情
貨號 | 產品名稱 | 規格 |
GTX627408-S | GAPDH antibody | 25 μl |
GTX627408 | 100 μl |
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